Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 555/山羊抗小鼠IgG (H+L) Superclonal™重组二抗，Alexa Fluor 555

货号：A28180

规格：1mg

价格：3337

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Recombinant full-length protein

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 555

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	1 mg/mL	用法：	2 μg/mL (ICC)；2 μg/mL (IF)

产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (A28180) in IFImmunofluorescence analysis of Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 555 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 555 (Product # A-28180) was used at a concentration of 2 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Secondary Antibody (A28180) in IFEndogenous Tubulin (red) was labeled with Mouse anti-Tubulin primary antibody (Product # 32-2500) (1 µg/mL) in U20S human osteocarcinoma cells and visualized using Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 555 conjugate (Product # A28180) (0.4 µg/mL, 1:2500). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Stem cell researchGeneration of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene.

技术参数

产品应用 ICC;IF

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17398.htm