Attune® Wash Solution is an optimized cleaning buffer that is used in the Attune® Acoustic Focusing Cytometer to maintain cleanliness of the fluidics lines, minimizing contamination or carryover.

Maintains System Cleanliness

Attune® Wash Solution has been formulated to remove cellular debris, prevent protein building up, and minimize dyes sticking to the fluidics lines of the system. It is recommended for use during the routine maintenance and automated shutdown procedures of the Attune® Acoustic Focusing Cytometer.

Convenient

The ready-to-use 1X wash solution was designed to eliminate the need for dilution prior to use, as well as access to a purified water system. The wash solution is stable for 30 days after the bottle is opened.

High Quality

The Attune® Wash Solution is prepared, filtered, and packaged in a sterile environment to ensure the highest quality. Each batch is traceable by its lot number designation and has been validated for use on the Attune® Acoustic Focusing Cytometer.

Achieve functional knockout in human primary T-cells

T cell receptor knockout using TrueGuide Synthetic gRNAs. Human T cells were isolated and activated using Dynabeads magnetic beads (Cat. Nos. 11344D and 11141D, respectively) and then transfected with TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA for T cell receptor alpha (TRAC) or beta (TRBC) regions using the Neon Transfection System. Following transfection, editing efficiency was measured by the GeneArt Cleavage Detection assay (a) or by measuring % T cell receptor negative (TCR–) cells using the Attune NxT Flow Cytometer (b). Cells analyzed by flow cytometry were stained with a T cell receptor–specific antibody conjugated to PE (c).