DYKDDDDK Tag Monoclonal Antibody (FG4R), HRP/FLAG标签抗体
货号：MA1-91878-HRP
规格：50 µL
价格：2753
产品类型：标签抗体
品牌：Thermo Fisher
物种：Tag
宿主：小鼠
抗体亚型：IgG
荧光染料：HRP
点击Invitrogen标签抗体集，查看更多标签抗体
抗体类型: | 单抗 | 同型对照： | Mouse / IgG2b |
免疫原性： | Synthetic peptide (DYKDDDDK) coupled to KLH. | 用法： | 1µg/mL（WB) |
Product Specific Informationsimilar epitope as the FLAG tag from SigmaMA1-91878 detects Flag tag from various samples.MA1-91878 has been successfully used in Western blot applications.The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.Background/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| DYKDDDDK Tag Antibody (MA1-91878-HRP) in WBWestern blot analysis of FLAG Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated FLAG Epitope Tag monoclonal antibody (Product # MA1-91878-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). |
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参考文献： |
| 1.Journal of lipid researchRegulation of the human prostacyclin receptor gene by the cholesterol-responsive SREBP1."MA1-91878-HRP was used in western blot to investigate the influence of cholesterol on human IP [(h)IP] expression"AuthorsTurner EC,Kinsella BT |
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