Tetramethylrhodamine, Ethyl Ester, Perchlorate (TMRE)
货号：T669
规格：25 mg
价格：3268
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| Tetramethylrhodamine, ethyl ester (TMRE) is a cell-permeant, cationic, red-orange fluorescent dye that is readily sequestered by active mitochondria. |
| 数据： |
| Detection of apoptosis in SK-N-MC neuroblastoma cells.Detection of apoptosis in SK-N-MC neuroblastoma cells. Following a six-hour exposure to hydrogen peroxide, cells were labeled with Hoechst 33342 (Cat. no. H1399, H3570, H21492), tetramethylrhodamine ethyl ester (TMRE, Cat. no. T669) and rhodamine 110, bis-L-aspartic acid amide (Cat. no. R22122) for 15 minutes. Apoptotic cells show green cytosolic fluorescence resulting from cleavage of the rhodamine 110, bis-L-aspartic acid amide substrate by active caspase-3. The staining pattern of the Hoechst 33342 dye reveals that the majority of the rhodamine 110–positive cells also contain condensed or fragmented nuclei characteristic of apoptosis. Furthermore, the rhodamine 110–positive cells are also characterized by an absence of polarized mitochondria, as indicated by their failure to load the positively charged mitochondrial indicator TMRE. The image was contributed by A.K. Stout and J.T. Greenamyre, Emory University. |
| 相关产品： |
| 细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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