DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 555/FLAG标签抗体

货号：MA1-142-A555

规格：50 µl

价格：4223

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：大鼠

抗体亚型：IgG

荧光染料：Alexa Fluor 555

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG2a
免疫原性：	DYKDDDDK-tagged mouse Langerin	用法：	1:25-1:100 (ICC);1:25-1:100（IF);1:500（WB)

Product Specific Informationsimilar epitope as the Flag tag from SigmaBackground/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

DYKDDDDK Tag Antibody (MA1-142-A555) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing a FLAG Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 555 conjugated FLAG-tag monoclonal antibody (Product # MA1-142-A555) at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. DYKDDDDK Tag Antibody (MA1-142-A555) in WB Western blot analysis of FLAG Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and blocked with Sea Block blocking buffer for at least 1 hour. The membrane was probed with a AlexaFluor555-conjugated FLAG Epitope Tag monoclonal antibody (Product # MA1-142-A555) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging system.

推荐产品：

二抗 荧光二抗 免疫组化一抗 标签抗体

参考文献：

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17529.htm