DYKDDDDK Tag Monoclonal Antibody (L5), Alexa Fluor 647/FLAG标签抗体
货号：MA1-142-A647
规格：50 µl
价格：3849
产品类型：标签抗体
品牌：Thermo Fisher
物种：Tag
宿主：大鼠
抗体亚型：IgG
荧光染料：Alexa Fluor 647
点击Invitrogen标签抗体集，查看更多标签抗体
抗体类型: | 单抗 | 同型对照： | IgG2a |
免疫原性： | DYKDDDDK-tagged mouse Langerin | 用法： | |
Product Specific Informationsimilar epitope as the Flag tag from Sigma.Background/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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