HA Tag Monoclonal Antibody (2-2.2.14), HRP/HA标签抗体

货号：26183-HRP

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：HRP

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	HA peptide YPYDVPDYA derivitized to ovalbumin.	用法：	1:500-1:2000（WB)

Background/Target InformationHA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

HA Tag Antibody (26183-HRP) in WBWestern blot analysis of HA Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated HA Epitope Tag monoclonal antibody (Product # 26183-HRP) at a dilution of 1:1000 overnight at 4°C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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参考文献：

1.PloS oneValproic acid causes proteasomal degradation of DICER and influences miRNA expression."26183-HRP was used in western blot to study the proteasomal degradation of DICER and altered miRNA levels induced by valproic acid"AuthorsZhang Z,Convertini P,Shen M,Xu X,Lemoine F,de la Grange P,Andres DA,Stamm S2.Molecular and cellular biologyFunctional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis."26183-HRP was used in western blot to study the role of TMAP/CKAP2 in mitotic spindle assembly and maintenance"AuthorsHong KU,Park YS,Seong YS,Kang D,Bae CD,Park J

技术参数

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