c-Myc Antibody (MA1-980) in Flow Flow cytometric analysis of c-Myc (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37°C. Cells were incubated with a c-Myc monoclonal antibody (Product # MA1-980) or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500 µL of FACS buffer containing 10 µL of 4% paraformaldehyde, and analyzed on a flow cytometer. c-Myc Antibody (MA1-980) in IF Immunofluorescent analysis of c-Myc (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Product # MA1-980) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification. c-Myc Antibody (MA1-980) in WB Western blot analysis of c-Myc was performed by loading 40 µg of HeLa or NCCIT nuclear fractions (NF) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with c-Myc Monoclonal Antibody, 9E10 (Product # MA1-980) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgG HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product # 34075). Nuclear fractions were generated using the NE-PER kit (Product # 78833). c-Myc Antibody (MA1-980) in IHC Immunohistochemistry was performed on cancer biopsies of deparaffinized Human lung squamous carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a Mouse Monoclonal Antibody recognizing c-myc (Product # MA1-980) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. c-Myc Antibody (MA1-980) in IHC (P) Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a Mouse Monoclonal Antibody recognizing c-myc (Product # MA1-980) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. |
1.The Journal of biological chemistry Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor activity-modifying protein 1 (RAMP1), and beta-arrestin. "MA1-980 was used in flow cytometry, immunocytochemistry, immunoprecipitation, and western blot to study oligomerization and localization of the calcitonin receptor-like receptor" AuthorsHilairet S,Bélanger C,Bertrand J,Laperrière A,Foord SM,Bouvier M 2.International journal of oncology Identification of prognostic biomarkers for glioblastomas using protein expression profiling. "MA1-980 was used in immunohistochemistry - paraffin section to identify diagnostic and prognostic markers for glioblastoma" AuthorsJung Y,Joo KM,Seong DH,Choi YL,Kong DS,Kim Y,Kim MH,Jin J,Suh YL,Seol HJ,Shin CS,Lee JI,Kim JH,Song SY,Nam DH 3.Nature communications Menin enhances c-Myc-mediated transcription to promote cancer progression. "MA1-980 was used in Western Blotting to identify an unexpected role of Menin in enhancing the transactivity of oncogene MYC in a way independent of H3K4me3 activity." AuthorsWu G,Yuan M,Shen S,Ma X,Fang J,Zhu L,Sun L,Liu Z,He X,Li T,Li C,Wu J,Hu X,Li Z,Song L,Qu K,Zhang H,Gao P 4.American journal of human genetics Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis. "MA1-980 was used in western blot to identify MAPKBP1 mutations as a genetic cause of juvenile or late-onset and cilia-independent nephronophthisis" AuthorsMacia MS,Halbritter J,Delous M,Bredrup C,Gutter A,Filhol E,Mellgren AEC,Leh S,Bizet A,Braun DA,Gee HY,Silbermann F,Henry C,Krug P,Bole-Feysot C,Nitschké P,Joly D,Nicoud P,Paget A,Haugland H,Brackmann D,Ahmet N,Sandford R,Cengiz N,Knappskog PM,Boman H,Linghu B,Yang F,Oakeley EJ,Saint Mézard P,Sailer AW,Johansson S,Rødahl E,Saunier S,Hildebrandt F,Benmerah A 5.Nature communications DELAY OF GERMINATION1 requires PP2C phosphatases of the ABA signalling pathway to control seed dormancy. "MA1-980 was used in Western Blotting to describe four phosphatases that interact with DOG1 in seeds." AuthorsNée G,Kramer K,Nakabayashi K,Yuan B,Xiang Y,Miatton E,Finkemeier I,Soppe WJJ |
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