c-Myc Monoclonal Antibody (9E10), HRP/c-Myc标签抗体

货号：MA1-980-HRP

规格：100 µL

价格：4050

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：HRP

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.	用法：	1:250-1:1000(WB)

Product Specific InformationMA1-980-HRP detects c-myc protein and c-myc tagged proteins.MA1-980-HRP has been successfully used in Western blot procedures.The MA1-980-HRP immunogen corresponds to the synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.Background/Target InformationThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun Nterminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

c-Myc Antibody (MA1-980-HRP) in WBWestern blot analysis of c-Myc was performed by loading 50 µg of NCCIT and Jurkat lysates, and 10 µg of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated c-Myc monoclonal antibody (Product # MA1-980-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 min (NCCIT and Jurkat) and 3 sec (Myc-MYCBP). Results show a band at ~57 kDa (NCCIT and Jurkat) and 16 kDa (Myc-MYCBP) as well as a 2 unknown bands at 35-40 kDa.

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