V5 Tag Monoclonal Antibody (E10/V4RR), DyLight 650/V5标签抗体

货号：MA5-15253-D650

规格：50 µL

价格：2875

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：DyLight 650

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH	用法：	1:25-1:100（IF）；1:25-1:100（ICC）

Product Specific InformationMA5-15253-D650 has been successfully used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672 nm.Background/Target InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST),although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

V5 Tag Antibody (MA5-15253-D650) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing a V5 Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 650-conjugated V5 Epitope Tag monoclonal antibody (Product # MA5-15253-D650) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

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参考文献：

1.Journal of virologyFunctional Comparison of Mx1 from Two Different Mouse Species Reveals the Involvement of Loop L4 in the Antiviral Activity against Influenza A Viruses.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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