TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), APC, eBioscience™

货号：17-8883-42

规格：100 tests

价格：3198

产品类型：流式抗体

品牌：eBioscience

抗原：TRA-1-81

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：TRA-1-81

荧光染料：APC

类型:	单抗	同型对照：	IgM
免疫原性：	human embryonal carcinoma cell line 2102Ep	用法：	5 µL (0.25 µg)/test(Flow)

Product Specific InformationDescription: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TRA-1-81 antibody has been pre-titrated and tested by flow cytometric analysis of 2102Ep human embryonal carcinoma cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-81 (Podocalyxin) Antibody (17-8883-42) in FlowStaining of the 2102Ep human embryonal carcinoma cell line with staining buffer (autofluorescence) (green histogram) or Anti-Human TRA-1-81 APC (purple histogram). Total viable cells were used for analysis.

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参考文献：

1.Stem cells and developmentSimplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.2.Stem cell reviewsComparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).3.Hepatology (Baltimore, Md.)Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells.

技术参数

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