V5 Tag Monoclonal Antibody (2F11F7)/V5标签抗体

货号：37-7500

规格：100 µg

价格：4050

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：IgG2a, kappa

荧光染料：其它

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：	sequence -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-	用法：	0.1-1.0 µg/mL（ELISA）；1.0 µg/mL(WB)

Background/Target InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST),although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

V5 Tag Antibody (37-7500) in IFImmunofluorescent analysis of V5 tag was done on HEK-293 cells transiently overexpressing V5-His -LacZ. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with V5 Tag Mouse Monoclonal Antibody (Product # 37-7500) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Panel d is a merged image showing cytoplasmic localization. Panel e is untransfected HEK-293 cells. The images were captured using a Nikon microscope at 20X magnification.

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参考文献：

1.AutophagyTranscription factor NFE2L2/NRF2 is a regulator of macroautophagy genes."37-7500 was used in ChIP assay to identify NFE2L2/NRF2 as a regulator of autophagy gene expression and study its role in Alzheimer disease"AuthorsPajares M,Jiménez-Moreno N,García-Yagüe ÁJ,Escoll M,de Ceballos ML,Van Leuven F,Rábano A,Yamamoto M,Rojo AI,Cuadrado A2.The Journal of cell biologyDivergent regulation of functionally distinct γ-tubulin complexes during differentiation."37-7500 was used in immunocytochemistry to study functionally distinct gamma-tubulin complexes and divergent regulation during differentiation"AuthorsMuroyama A,Seldin L,Lechler T3.The Journal of biological chemistryThe COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin.AuthorsBao X,Cai W,Deng H,Zhang W,Krencik R,Girton J,Johansen J,Johansen KM4.CellModeling familial cancer with induced pluripotent stem cells."37-7500 was used in western blot to generate patient-derived iPSCs from a Li-Fraumeni syndrome family and assess the role of mutant p53 in the development of osteosarcoma"AuthorsLee DF,Su J,Kim HS,Chang B,Papatsenko D,Zhao R,Yuan Y,Gingold J,Xia W,Darr H,Mirzayans R,Hung MC,Schaniel C,Lemischka IR5.The Journal of biological chemistryProteolytic Processing of Neuregulin 1 Type III by Three Intramembrane-cleaving Proteases."377500 was used in immunoprecipitation to characterize the proteolytic cleavage of neuregulin 1 type III"AuthorsFleck D,Voss M,Brankatschk B,Giudici C,Hampel H,Schwenk B,Edbauer D,Fukumori A,Steiner H,Kremmer E,Haug-Kröper M,Rossner MJ,Fluhrer R,Willem M,Haass C

技术参数

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