V5 Tag Monoclonal Antibody (2F11F7), Alexa Fluor 488/V5标签抗体

货号：37-7500-A488

规格：50 µl

价格：3405

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：Alexa Fluor 488

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：	sequence -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-	用法：	1-500(WB)

Background/Target InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST),although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

V5 Tag Antibody (37-7500-A488) in WBWestern blot analysis of V5 Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and blocked with Sea Block blocking buffer for at least 1 hour. The membrane was probed with a AlexaFluor488-conjugated V5 Epitope Tag monoclonal antibody (Product # 37-7500-A488) at a dilution of 1:500 over night at 4C on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging system.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.eLifeProgerin reduces LAP2α-telomere association in Hutchinson-Gilford progeria.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.Cancer researchGenetic alterations in the phosphoinositide 3-kinase/Akt signaling pathway confer sensitivity of thyroid cancer cells to therapeutic targeting of Akt and mammalian target of rapamycin.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.PloS oneCharacterization of FUS mutations in amyotrophic lateral sclerosis using RNA-Seq.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17586.htm