TurboGFP Polyclonal Antibody/TurboGFP抗体
货号：PA5-22688
规格：100 µg
价格：5442
产品类型：标签抗体
品牌：Thermo Fisher
物种：Tag
宿主：兔
抗体亚型：IgG
荧光染料：其它
抗体类型: | 多抗 | 同型对照： | IgG |
免疫原性： | Full-length recombinant denatured TurboGFP and non-denatured CopGFP comprising 6XHis Tag | 用法： | 1:30,000-1:50,000（ELISA）；1:1000-1:5000(WB)；1:1000-1:5000(ICC);1:1000-1:5000(IF) |
Product Specific InformationPA5-22688 can be used to recognize denatured and non-denatured TurboGFP and CopGFP in immunocytochemistry, Western blot, and ELISA assays.TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda)Reconstitute with sterile water or 50% glycerol to a concentration of 1 mg/mL. Store at -20 C for one year; reconstituted with sterile water, antibody can be stored at +2 - +8°C for threemonths; reconstituted with 50% glycerol, antibody can be stored at -20°C for six months.Background/Target InformationThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| TurboGFP Antibody (PA5-22688) in IFImmunofluorescence analysis of TurboGFP expression in HeLa cells shown in brightfield, TurboGFP expression (green), and using a polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:3000 followed by an anti-rabbit secondary antibody (red).TurboGFP Antibody (PA5-22688) in WBWestern blot analysis of recombinant TurboGFP was performed by loading 50 ng of purified protein onto a 14% SDS-PAGE gel. Recombinant protein was purified from transformed E. coli and the sample was boiled before loading. After transfer, membranes were probed with a rabbit polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:20,000. Membranes were probed with a goat anti-rabbit-HRP secondary antibody and then detected by chemiluminescent (ECL) or colorimetric (TMB) detection. |
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参考文献： |
| 1.PloS oneCytocidal activities of topoisomerase 1 inhibitors and 5-azacytidine against pheochromocytoma/paraganglioma cells in primary human tumor cultures and mouse cell lines."PA5-22688 was used in western blot to study the therapeutic potential of topoisomerase inhibitors and 5-azacytidine aginst metastatic pheochromocytoma and paraganglioma"AuthorsPowers JF,Korgaonkar PG,Fliedner S,Giubellino A,Pacak K,Sahagian GG,Tischler AS2.Cellular and molecular gastroenterology and hepatologyMitogen-activated Protein Kinase Kinase Activity Maintains Acinar-to-Ductal Metaplasia and Is Required for Organ Regeneration in Pancreatitis."PA5-22688 was used in immunohistochemistry and western blot to investigate the role of mitogen-activated protein kinase in pancreatitis"AuthorsHalbrook CJ,Wen HJ,Ruggeri JM,Takeuchi KK,Zhang Y,di Magliano MP,Crawford HC3.Nature structural and molecular biologyEarly embryonic-like cells are induced by downregulating replication-dependent chromatin assembly."PA5-22688 was used in immunocytochemistry to investigate how early embryonic-like cells are promoted by downregulating replication-dependent chromatin assembly"AuthorsIshiuchi T,Enriquez-Gasca R,Mizutani E,Bošković A,Ziegler-Birling C,Rodriguez-Terrones D,Wakayama T,Vaquerizas JM,Torres-Padilla ME |
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