beta Galactosidase Monoclonal Antibody (DC1 4C7)/β-Gal 抗体

货号：MA1-152

规格：100 µg

价格：5441

产品类型：抗体和染料

品牌：Thermo Fisher

物种：其它

宿主：小鼠

抗体亚型：其它

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	Full length E. coli beta Galactosidase	用法：	1:50-1:100（ICC）；1:50-1:100（IF）；1:500 (WB)

Product Specific InformationWestern blot analysis of MA1-152 detects recombinant E. coli beta Galactosidase at ~110 kDa. This antibody also detects E. coli beta Galactosidase in pCMV-lacZ transfected HeLa cells.Background/Target InformationBeta Galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety. Deficiencies in the protein in humans can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. E. coli beta Galactosidase is commonly used in molecular biology as a reporter marker tomonitor gene expression. Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. Beta Galactosidase can be split in two peptides, lacZalpha and LacZOmega, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors. The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others. Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Galactosidase Antibody (MA1-152) in IFImmunofluorescent analysis of E. coli beta-Galactosidase (green) in pCMV-LacZ transfected HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a beta-Galactosidase monoclonal antibody (Product # MA1-152) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. beta Galactosidase Antibody (MA1-152) in WBWestern blot analysis of beta-Galactosidase was performed by loading various amounts of full length recombinant E. coli beta-Galactosidase and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. The membrane was probed with a beta-Galactosidase monoclonal antibody (Product # MA1-152) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1% Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). beta Galactosidase Antibody (MA1-152)Microbial cell factories 2015 - Fig. 4 Comparison of activities of free and membrane bound beta-gal-cyt b 5 . The activity of beta-gal-cyt b 5 immobilized to SUV ( a ) and the activity of the same samples after solubilization of beta-gal-cyt b 5 by detergent treatment ( b ) are compared. The reference activity ( c ) was obtained from purified beta-galactosidase with N-terminal His 6 -tagView full figure onBy clicking this image or link above, you will be redirected to the Benchsci website.Disclaimer

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参考文献：

1.Microbial cell factoriesA novel one-step expression and immobilization method for the production of biocatalytic preparations.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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