| 产品介绍 | | |
| Rat anti Human CD52 antibody, clone YTH34.5recognizes the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small but heavily glycosylated peptide attached to the cell surface membrane via a GPI link (Xiaet al.1991).The apparent molecular mass of the native antigen on SDS-PAGE is 25-29 kDa, considerably reduced following N-glycanase treatment (Rowanet al.1998).CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Humanized versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Deardenet al.2002;Pettittet al.2012). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Human monocytes |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | 1/20 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | F | 1ml |
| 文献 |
| 1.Klangsinsirikul, P.et al.(2002) Campath-1G causes rapid depletion of circulating host dendritic cells (DCs) before allogeneic transplantation but does not delay donor DC reconstitution.Blood. 99: 2586-91.2.Ratzinger, G.et al.(2003) Differential CD52 expression by distinct myeloid dendritic cell subsets: implications for alemtuzumab activity at the level of antigen presentation in allogeneic graft-host interactions in transplantation.Blood. 101: 1422-9.3.Zand, M.S.et al.(2005) A renewable source of donor cells for repetitive monitoring of T- and B-cell alloreactivity.Am J Transplant. 5: 76-86. |
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