GFAP Monoclonal Antibody (2.2B10)/胶质纤维酸性蛋白抗体

货号：13-0300

规格：100 µg

价格：4793

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：	Enriched bovine glial filaments.	用法：	0.1-0.5 µg/mL（ELISA）；10-50 µg/mL（IHC）；2-5 µg（IP）

Background/Target InformationGFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily, and specifically, expressed in astrocytes and certain astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GFAP Antibody (13-0300) in IHCImmunohistochemical staining of astrocytoma tissue using Rt anti-GFAP (Product # 13-0300). GFAP Antibody (13-0300) in IFImmunofluorescent analysis of GFAP in human iPSC-derived astrocytes. The cells were fixed with 4% paraformaldehyde for 15 min at room temperature, and then permeabilized and blocked with 0.25% Triton X-100 and 10% donkey serum in PBS for 20 min. Samples were then incubated with a Mouse GFAP monoclonal antibody (green; Product # MA5-12023) at a dilution of 1:500 in PBS containing 0.25% Triton X-100 and 10% donkey serum at 48304C overnight, followed by incubation with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114) at a dilution of 1:1000 as well as DAPI (blue; 1:25000) in PBS containing 0.25% Triton X-100 and 10% donkey serum at room temperature for 1 hour. Images were taken at 40X magnification. Data courtesy of Dr. Zhexing Wen at Emory University.

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参考文献：

1.Nature communicationsReactive astrocytes function as phagocytes after brain ischemia via ABCA1-mediated pathway."13-0300 was used in Immunohistochemistry to show that following brain ischemia, reactive astrocytes become phagocytic and engulf debris via the ABCA1 pathway."AuthorsMorizawa YM,Hirayama Y,Ohno N,Shibata S,Shigetomi E,Sui Y,Nabekura J,Sato K,Okajima F,Takebayashi H,Okano H,Koizumi S2.Journal of neurovirologyPhrenic nerve deficits and neurological immunopathology associated with acute West Nile virus infection in mice and hamsters."130300 was used in immunohistochemistry to test if the respiratory deficits in West Nile virus-infected rodents are neurological"AuthorsZukor K,Wang H,Hurst BL,Siddharthan V,Van Wettere A,Pilowsky PM,Morrey JD3.The Journal of comparative neurologyRetinal ganglion cell survival and axon regeneration after optic nerve injury in naked mole-rats."13-0300 was used in immunohistochemistry to elucidate optic nerve injury in naked mole-rats and axon regeneration and retinal ganglion cell survival"AuthorsPark KK,Luo X,Mooney SJ,Yungher BJ,Belin S,Wang C,Holmes MM,He Z4.The Journal of neuroscience : the official journal of the Society for NeuroscienceMultimodal Imaging for DREADD-Expressing Neurons in Living Brain and Their Application to Implantation of iPSC-Derived Neural Progenitors."130300 was used in immunohistochemistry to use clozapine-N-oxide a functional reporter probe for positron emission tomography to study designer receptor exclusively activated by designer drugs in living brains"AuthorsJi B,Kaneko H,Minamimoto T,Inoue H,Takeuchi H,Kumata K,Zhang MR,Aoki I,Seki C,Ono M,Tokunaga M,Tsukamoto S,Tanabe K,Shin RM,Minamihisamatsu T,Kito S,Richmond BJ,Suhara T,Higuchi M5.Neurobiology of diseaseSTAT3 and SOCS3 regulate NG2 cell proliferation and differentiation after contusive spinal cord injury."13-0300 was used in Immunohistochemistry to identify GFAP astrocytes in NG2 cell proliferation, to understand the mechanism of STAT3 and SOCS3 regulation in response to CNS injury."AuthorsHackett AR,Lee DH,Dawood A,Rodriguez M,Funk L,Tsoulfas P,Lee JK

技术参数

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