| beta Actin Loading Control Antibody (MA5-15739) in WB1:1000 (1 µg/mL) Ab dilution used in WB of 20 µg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)beta Actin Loading Control Antibody (MA5-15739) in FlowFlow cytometry analysis of Beta Actin in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Actin loading control antibody (Product # MA5-15739) at a dilution of 2 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.beta Actin Loading Control Antibody (MA5-15739) in IHC (P)Immunohistochemistry analysis of Beta Actin showing staining in the cytoskeleton of paraffin-embedded mouse skeletal muscle tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Beta Actin loading control antibody (Product # MA5-15739) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. beta Actin Loading Control Antibody (MA5-15739) in IFImmunofluorescence analysis of beta Actin was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Actin (BA3R) Loading Control Mouse Monoclonal Antibody (Product # MA5-15739-1MG) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. |
| 1.Frontiers in immunologyLipopolysaccharide Attenuates Induction of Proallergic Cytokines, Thymic Stromal Lymphopoietin, and Interleukin 33 in Respiratory Epithelial Cells Stimulated with PolyI:C and Human Parechovirus."MA5-15739 was used in Western Blotting to examine the effect of lipopolysaccharide on the expression of the proallergic cytokines thymic stromal lymphopoietin and interleukin 33 in H292 cells."AuthorsLin TH,Cheng CC,Su HH,Huang NC,Chen JJ,Kang HY,Chang TH2.Cell death discoveryFoxO1 interacts with transcription factor EB and differentially regulates mitochondrial uncoupling proteins via autophagy in adipocytes."MA5-15739 was used in western blot to elucidate how FoxO1 regulates mitochondrial uncoupling proteins"AuthorsLiu L,Tao Z,Zheng LD,Brooke JP,Smith CM,Liu D,Long YC,Cheng Z3.Molecular neurobiologyPDGF-BB Preserves Mitochondrial Morphology, Attenuates ROS Production, and Upregulates Neuroglobin in an Astrocytic Model Under Rotenone Insult."MA515739 was used in western blot to discuss mechanisms associated with platelet-derived growth factor, subtype BB protection against oxidative damage"AuthorsCabezas R,Vega-Vela NE,González-Sanmiguel J,González J,Esquinas P,Echeverria V,Barreto GE4.Scientific reportsThe c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells."MA5-15739 was used in Western Blotting to suggest that an ancestral response, involving the JNK pathway, is remarkably conserved from corals to human, protecting cells from the adverse environmental effects."AuthorsCourtial L,Picco V,Grover R,Cormerais Y,Rottier C,Labbe A,Pagès G,Ferrier-Pagès C5.Oxidative medicine and cellular longevityCoordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment."MA5-15739 was used in western blot to study mitochondrial biogenesis and mitophagy in cancer cells"AuthorsZou P,Liu L,Zheng LD,Payne KK,Manjili MH,Idowu MO,Zhang J,Schmelz EM,Cheng Z |
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