beta Actin Loading Control Monoclonal Antibody (BA3R), Biotin

货号：MA5-15739-BTIN

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Biotin

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类型:	单抗	同型对照：	IgG2b
免疫原性：	Beta-actin N-terminal peptide.	用法：	1-5µg/test(flow);1 µg/mL(WB)

Product Specific InformationMA5-15739-BTIN has been successfully used for Western blot and FACS applications.Background/Target InformationBeta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Actin Loading Control Antibody (MA5-15739-BTIN) in FlowFlow cytometry analysis of Beta Actin in A431 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Actin loading control antibody (Product # MA5-15739) at a dilution of 2 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis. beta Actin Loading Control Antibody (MA5-15739-BTIN) in WBWestern blot analysis of Beta-Actin was performed by loading 30 µg of various whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated Beta-Actin monoclonal antibody (Product # MA5-15739-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

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参考文献：

1.PloS oneEffects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex."MA5-15739-BTIN was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats"AuthorsCastro B,Sánchez P,Torres JM,Ortega E2.Annals of neurologyAmylin deposition in the brain: A second amyloid in Alzheimer disease?"MA5-15739-BTIN was used in western blot to study the formation of amylin amyloid deposits in the cerebrovascular system and the implications for Alzheimer's disease"AuthorsJackson K,Barisone GA,Diaz E,Jin LW,DeCarli C,Despa F3.Nature biotechnologyA comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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