beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 550
货号：MA5-16308-D550
规格：50 µL
价格：2753
产品类型：内参抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：其它
荧光染料：DyLight 550
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类型: | 单抗 | 同型对照： | IgG2a |
免疫原性： | KLH conjugated peptide of the N-terminus of Beta-Tubulin. | 用法： | 1:25-1:100(ICC);1:25-1:100(IF) |
Product Specific InformationMA5-16308-D550 has successfully been used for immunofluorescence. DyLight 550 has an excitation/emission of 562/576nm.Background/Target InformationBeta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| beta Tubulin Loading Control Antibody (MA5-16308-D550) in IFImmunofluorescent analysis of Beta-Tubulin (red) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 550-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-D550) at a dilution of 1:50 for at least 1 hour at room temperature. F-actin (green) was stained with DyLight 488 Phalloidin (Product # 21833) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification. |
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