ZO-1 Monoclonal Antibody (ZO1-1A12)

货号： 33-9100

规格：100 µg

价格：4793

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：其它

荧光染料：其它

类型:	单抗	同型对照：	IgG1
免疫原性：	Human recombinant ZO-1 fusion protein encompassing amino acids 334-634	用法：	0.1-1.0 µg/mL(ELISA);5-10 µg/mL(IF);1-2 µg/mL

Product Specific Information33-9100 has been successfully used in ELISA, Immunofluorescence and Western Blot analysis of ZO-1.Target InformationZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

ZO-1 Antibody (33-9100) in IFMDCK canine cells stained with Mouse anti-ZO-1 (Product # 33-9100) and Zymed's ZyMAX FITC-Goat Anti-Mouse IgG (H+L) (Product # 81-6511). ZO-1 Antibody (33-9100) in IFImmunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without ZO-1 mouse monoclonal antibody (Product # 33-9100), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour s at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. ZO-1 Antibody (33-9100) in IFImmunofluorescent analysis of ZO-1 (red) and SOX2 (grey) in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse ZO-1 monoclonal antibody (red; Product # 33-9100) at a dilution of 1:500, a Rabbit SOX2 polyclonal antibody (grey; Product # PA1-094X) at a dilution of 1:500, and a Chicken MAP2 polyclonal antibody (green) at a dilution of 1:1000 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 568 (Product # A10037), Donkey anti-Rabbit Alexa Fluor 647 (Product # A-31573), and Donkey Anti-Chicken Alexa Fluor 488 at a dilution of 1:1000 as well as DAPI (blue; 1:25000) in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm. Data courtesy of Dr. Zhexing Wen at Emory University.

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参考文献：

1.Nature communicationsWnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.Cellular and molecular gastroenterology and hepatologyHuman Fetal-Derived Enterospheres Provide Insights on Intestinal Development and a Novel Model to Study Necrotizing Enterocolitis (NEC).View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.Frontiers in molecular neuroscienceGlial Cells in the Fish Retinal Nerve Fiber Layer Form Tight Junctions, Separating and Surrounding Axons.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4.BladderThe human urothelial tight junction: claudin 3 and the ZO-1α+switch.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5.Nature communicationsCDC20B is required for deuterosome-mediated centriole production in multiciliated cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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