ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 488
货号：MA3-39100-A488
规格：50 µl
价格：4223
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：其它
荧光染料：Alexa Fluor 488
类型: | 单抗 | 同型对照： | IgG1 |
免疫原性： | Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 | 用法： | 1-10 µg/mL(ICC);1-10 µg/mL(IF) |
Target InformationZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| ZO-1 Antibody (MA3-39100-A488) in IFImmunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA3-39100-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
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