Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor 647

货号： MA1-065-A647

规格：50 µl

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：其它

荧光染料：Alexa Fluor 647

类型:	单抗	同型对照：	IgG1
免疫原性：	Purified human brain clathrin heavy chain.	用法：	1-10 µg/mL(IF);1-10 µg/mL(ICC)

Product Specific InformationMA1065A647 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.The MA1065A647 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1065A647 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.Target InformationClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Clathrin Heavy Chain Antibody (MA1-065-A647) in IFImmunofluorescent analysis of Clathrin Heavy Chain (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Clathrin Heavy Chain Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA1-065-A647) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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