PMP70 Polyclonal Antibody

货号：PA1-650

规格：100 µg

价格：5618

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659)	用法：	0.5 µg/test（test）;1-10 µg/mL(IF);1:500(WB)

Product Specific InformationPA1-650 detects peroxisomal membrane protein 70 (PMP70) from mouse tissues.PA1-650 has been successfully used in Western blot, flow cytometry, and immunofluorescence procedures. By Western blot, this antibody detects an ~70 kDa protein representing PMP70 from peroxisome-enriched fractions from L cells.PA1-650 immunizing peptide corresponds to amino acid residues 644-659 from rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. PA1-650 immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.Target InformationPeroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum's disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

PMP70 Antibody (PA1-650) in IFImmunofluorescent analysis of PMP70 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PMP70 rabbit polyclonal antibody (Product # PA1-650), at a dilution of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. PMP70 Antibody (PA1-650) in FlowFlow cytometry analysis of PMP70 in 293T cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PMP70 polyclonal antibody (Product # PA1-650) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.PLoS pathogensIntegrated systems biology analysis of KSHV latent infection reveals viral induction and reliance on peroxisome mediated lipid metabolism."PA1650 was used in flow cytometry and immunocytochemistry to characterize the transcriptome of Kaposi's Sarcoma associated Herpesvirus infected cells"AuthorsSychev ZE,Hu A,DiMaio TA,Gitter A,Camp ND,Noble WS,Wolf-Yadlin A,Lagunoff M2.EMBO reportsPeroxisomal protein PEX13 functions in selective autophagy."PA1-650 was used in immunocytochemistry to study the role of PEX13 in autophagy"AuthorsLee MY,Sumpter R,Zou Z,Sirasanagandla S,Wei Y,Mishra P,Rosewich H,Crane DI,Levine B3.EndocrinologyPeroxisomal localization of the proopiomelanocortin-derived peptides beta-lipotropin and beta-endorphin."PA1-650 was used in western blot to study the sub-cellular localisation of beta-lipotropin and beta-endorphin."AuthorsHöftberger R,Kunze M,Voigtländer T,Unterberger U,Regelsberger G,Bauer J,Aboul-Enein F,Garzuly F,Forss-Petter S,Bernheimer H,Berger J,Budka H4.The Journal of biological chemistrySmall interfering RNA knockdown of calcium-independent phospholipases A2 beta or gamma inhibits the hormone-induced differentiation of 3T3-L1 preadipocytes."PA1-650 was used in immunocytochemistry and western blot to study the effect of calcium-independent phospholipases A2 beta or gamma on 3T3-L1 preadipocyte differentiation"AuthorsSu X,Mancuso DJ,Bickel PE,Jenkins CM,Gross RW5.American journal of physiology. Gastrointestinal and liver physiologyPeroxisome proliferator-activated receptor-alpha regulates postischemic liver injury."PA1-650 was used in western blot to study the regulation of postischemic liver injury by PPAP alpha"AuthorsOkaya T,Lentsch AB

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17687.htm