Cytochrome C Monoclonal Antibody (6H2.B4)

货号：33-8200

规格：100 µg

价格：4809

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Rat Cytochrome C.	用法：	0.1-1 µg/mL（ELISA）;5-20 µg/mL(IF); 15µg(IP)

Product Specific InformationThis antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.Target InformationCytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Cytochrome C Antibody (33-8200) in IFImmunofluorescent analysis of Cytochrome C (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a Cytochrome C mouse monoclonal antibody (Product # 33-8200) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst Dye (Product # 62249). Images were taken at 20X magnification on a Thermo Scientific ToxInsight HCS imaging instrument. Orthogonal methodThis Antibody was verified by Orthogonal method to ensure that the antibody binds to the antigen stated.Cytochrome C Antibody (33-8200)The specificity of anti-Cytochrome C monoclonal antibody (Product # 33-8200) was demonstrated by the immunofluorescence detection of cytochrome C (green) co-localization with mitochondria stained with MitoTracker® Red CMXRos (Product # M-7512) in HeLa cells. Orthogonal method validation info.

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参考文献：

1.The Journal of biological chemistryElevated AKT activity protects the prostate cancer cell line LNCaP from TRAIL-induced apoptosis.AuthorsNesterov A,Lu X,Johnson M,Miller GJ,Ivashchenko Y,Kraft AS2.Journal of neurochemistryParkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies."33-8200 was used in immunocytochemistry to report the generation of fibrillar and vesicular inclusions in a long-term cybrid cell culture model used to study Parkinson's disease."AuthorsTrimmer PA,Borland MK,Keeney PM,Bennett JP,Parker WD3.Journal of cell scienceMitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.AuthorsLove DC,Kochan J,Cathey RL,Shin SH,Hanover JA,Kochran J4.The Journal of cell biologyThe Toxoplasma gondii protein ROP2 mediates host organelle association with the parasitophorous vacuole membrane.AuthorsSinai AP,Joiner KA

技术参数

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