HSP60 Monoclonal Antibody (4B9/89), Alexa Fluor 647
货号：MA3-012-A647
规格：50 µl
价格：4070
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：其它
荧光染料：Alexa Fluor 647
类型: | 单抗 | 同型对照： | IgG2a |
免疫原性： | Human placental HSP60. | 用法： | 1-10 µg/mL(IF);1-10 µg/mL(ICC) |
Product Specific InformationThe MA3012A647 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds either between amino acids 335-366 or 484-547.Target InformationHeat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| HSP60 Antibody (MA3-012-A647) in IFImmunofluorescent analysis of Hsp60 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Hsp60 Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-012-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-17711.htm
QQ:1749072012