Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488/山羊抗小鼠IgG (H+L)二抗,DyLight 488

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Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488/山羊抗小鼠IgG (H+L)二抗,DyLight 488

货号:35502

规格:1 mL

价格:2335

产品类型:荧光二抗

品牌:Thermo Fisher

抗原:Purified Mouse IgG, whole molecule

物种:小鼠

宿主:山羊

抗体亚型:IgG

荧光染料:DyLight 488

抗体类型:荧光二抗同型对照:
浓度: 1 mg/mL用法:

1:25 - 1:100(Flow);

1 µg/mL(ICC);

1 µg/mL(IF);1:50-1:2,000 (IHC)Assay Dependent (IHC(P));

Assay Dependent (IP);

1:5,000-1:20,000 (WB)

产品详细信息

Product # 35502 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 35502 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 488 Amax= 493 nm; Emax= 518 nm. Mole Dye/Mole Protein Ratio is lot-dependent.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (35502) in IFImmunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody DyLight® 488 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Secondary Antibody DyLight® 488 conjugate (Product # 35502) was used at a concentration of 1 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

Mouse IgG (H+L) Secondary Antibody (35502) in IF Immunofluorescent analysis of Phalloidin (dark red) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

Mouse IgG (H+L) Secondary Antibody (35502) in IFImmunofluorescent analysis of Phalloidin (orange) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/mL final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

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参考文献:

1. ACS synthetic biologyCyanobacterial Surface Display System Mediates Engineered Interspecies and Abiotic Binding.2. Biochemical and biophysical research communicationsAgrimol B suppresses adipogenesis through modulation of SIRT1-PPAR gamma signal pathway.3. Oncology lettersMitofusin-2 prevents skeletal muscle wasting in cancer cachexia.4. Human molecular geneticsMethod for widespread microRNA-155 inhibition prolongs survival in ALS-model mice.5. Nature chemical biologySelective class IIa histone deacetylase inhibition via a nonchelating zinc-binding group.

技术参数

产品应用 Flow;ICC;IHC;IF;IHC(P);IP;WB

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