KDEL Polyclonal Antibody

货号：PA1-013

规格：100 µg

价格：5468

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94	用法：	1-20 µg/mL(Flow)；8-16 µg/mL(WB);1:100-200(IHC)

Product Specific InformationPA1-013 detects KDEL from human, rat, mouse and hamster.PA1-013 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ~94 kDa protein representing GRP94 in mouse myeloma cells. Immunofluorescence staining of GRP78 in mouse myeloma cells with PA1-013 results in diffuse cytoplasmic staining consistent with specific ER localization. PA1-013 immunoprecipitates PDI, GRP78, an ~72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ~94 kDa protein representing GRP94 from numerous rodent tissues.The PA1-013 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.Reconstitute with PBS.Target InformationThe sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

KDEL Antibody (PA1-013) in IHCImmunohistochemistry was performed on normal biopsies of deparaffinized Mouse liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing KDEL (Product # PA1-013) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. KDEL Antibody (PA1-013) in IFImmunofluorescent analysis of KDEL in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a KDEL polyclonal antibody (Product # PA1-013) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). KDEL staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. KDEL Antibody (PA1-013) in FlowFlow cytometry analysis of KDEL/ER was done on HeLa cells. Cells were fixed, permeabilized and stained with a KDEL/ER rabbit polyclonal antibody (Product # PA1-013, purple histogram) or a rabbit IgG isotype control (Product # MA5-16384, black histogram) at a dilution of 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27040) at a dilution of 1:50 for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

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参考文献：

1.Proceedings of the National Academy of Sciences of the United States of AmericaAxons provide the secretory machinery for trafficking of voltage-gated sodium channels in peripheral nerve."PA1-013 was used in immunocytochemistry to characterize the secretory machinery in axons and study its contribution to plasma membrane delivery of sodium channels"AuthorsGonzález C,Cánovas J,Fresno J,Couve E,Court FA,Couve A2.The Journal of biological chemistryPrimary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.FASEB journal : official publication of the Federation of American Societies for Experimental BiologyChanges of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice."PA1-013 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice"AuthorsPapp E,Száraz P,Korcsmáros T,Csermely P4.Journal of lipid researchFurther characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies."PA1-013 was used in immunocytochemistry to investigate related factors affecting recombinant human ceramide kinase's activity"AuthorsVan Overloop H,Gijsbers S,Van Veldhoven PP5.Nature cell biologyFunctional proteomic screens reveal an essential extracellular role for hsp90 alpha in cancer cell invasiveness."PA1-013 was used in immunoprecipitation to study the role of extracellular Hsp90-alpha in the activation of MMP2 and invasiveness of fibrosarcoma cells"AuthorsEustace BK,Sakurai T,Stewart JK,Yimlamai D,Unger C,Zehetmeier C,Lain B,Torella C,Henning SW,Beste G,Scroggins BT,Neckers L,Ilag LL,Jay DG

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