Rabbit anti ATG16 (Internal Region) antibody
货号:AHP1894
规格:0.1 mg
价格:5287
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:ATG16 (Internal Region)
物种:人
宿主:兔
抗体亚型:IgG
荧光染料:Purified
产品介绍 | | |
Rabbit anti ATG16 (Internal Region) antibodyrecognizes human Autophagy-related protein 16 (ATG16).ATG16 is a member of the autophagy protein family and forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-Terminal domain, it has been suggested that these may form a platform for further protein-protein interactions.Multiple isoforms of ATG16 exist. |
产品详情 | | |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
Mouse | √ |
Rat | √ |
N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human ATG16 was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Immunogen | Synthetic peptide corresponding to a 18 aa sequence from near the amino terminus of human ATG16. |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Frozen | √ | | |
Western Blotting | √ | 0.25 | 0.5ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western BlottingAHP1894 detects a band of approximately 75 kDa in HeLa cell lysate. |
数据 |
Immunocytochemical staining of HeLa cells with Rabbit anri Human ATG16 (AHP1894).Western blot analysis of whole cell lysate from HeLa adenocarcinoma cells probed with Rabbit anti Human ATG16 (AHP1894) at 0.25(A) and 0.5(B) ug/ml. |
相关试剂 |
Description | Product Code | Applications | Pack Size |
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB | 0.5 mL |
技术参数 产品应用 C;WB
产品类型 Polyclonal Antibody
推荐用于 C;WB
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