Propidium Iodide - FluoroPure™ Grade
货号：P21493
规格：100mg
价格：2598
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
Because it is manufactured at our ISO 9001—certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC. |
| 数据： |
Detection of BrdU-incorporating cells using the SBIP technique.Detection of BrdU-incorporating cells using the SBIP (Strand Breaks Induced by Photolysis) technique. Exponentially growing human promyelocytic leukemia (Cat. no. HL60) cells were incubated with 20 µM BrdU (Cat. no. B23151) for 40 minutes and then with 20 µg/mL Hoechst 33258 (Cat. no. H1398, H3569, H21491) in the presence of 2% DMSO for an additional 20 minutes. After this incubation, the cells were exposed to 300 nm UV light for 5 minutes to selectively photolyze DNA that contained the incorporated BrdU, and then fixed in 70% ethanol. Subsequent incubation of the permeabilized cells with the ChromaTide® BODIPY® FL-14-dUTP (Cat. no. C7614) in the presence of exogenous terminal deoxynucleotidyl transferase resulted in incorporation of the fluorophore into DNA strand breaks, thereby labeling the S-phase cells. The DNA was counterstained with the red-fluorescent nucleic acid stain propidium iodide (Cat. no. P1304MP, P3566, P21493); therefore, the BODIPY® FL dye–labeled DNA appears yellow. The image was contributed by Zbigniew Darzynkiewicz, Cancer Research Institute, New York Medical College. |
| 相关产品： |
| ▪细胞周期检测▪细胞凋亡检测▪细胞增殖活性检测 |
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