Calnexin Monoclonal Antibody (AF18), Alexa Fluor 555

货号：MA3-027-A555

规格：50 µl

价格：4223

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Alexa Fluor 555

类型:	单抗	同型对照：	IgG1
免疫原性：	Focus human hepatoma cell lysate	用法：	1-10 µg/mL(IF)

Product Specific InformationMA3027A555 detects calnexin from human and mouse tissues.The MA3027A555 antigen is Focus human hepatoma cell lysate.Target InformationCalnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER.The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Calnexin Antibody (MA3-027-A555) in IFImmunofluorescent analysis of Calnexin (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Calnexin Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-027-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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