PDI Monoclonal Antibody (RL90)

货号：MA3-019

规格：100 µL

价格：5618

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

类型:	单抗	同型对照：	IgG2a
免疫原性：	Purified rat PDI protein.	用法：	1:100(IF);1:100(ICC);1:200-1:2000(WB)；0.5-1 µg/test（Flow）

Product Specific InformationMA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.MA3-019 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. MA3-019 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.The MA3-019 antigen is purified rat PDI.Target InformationThe three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

PDI Antibody (MA3-019) in IHCImmunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PDI (Product # MA3-019) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. PDI Antibody (MA3-019) in FlowFlow cytometry analysis of PDI in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis. PDI Antibody (MA3-019) in IFImmunofluorescent analysis of PDI (red) in MDCK cells. The cells were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a PDI mouse monoclonal antibody (Product # MA3-019), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A27027) at a dilution of 1:1000 for 30 minutes at room temperature (red). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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参考文献：

1.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismEmbryonic lethal abnormal vision proteins and adenine and uridine-rich element mRNAs after global cerebral ischemia and reperfusion in the rat."MA3019 was used in western blot to study HuR, HuB, HuC, and HuD in hippocampal CA1 neurons"AuthorsWang H,Tri Anggraini F,Chen X,DeGracia DJ2.The Journal of cell biologyOxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins."MA3-019 was used in western blot to determine how N-glycosylation of cysteine-proximal acceptor sites in glycoproteins requires oxidoreductase activity"AuthorsCherepanova NA,Shrimal S,Gilmore R3.PloS oneEffects of oxidative stress on the solubility of HRD1, a ubiquitin ligase implicated in Alzheimer's disease."MA3-019 was used in western blot to study the effects of oxidative stress on the solubility of the Alzheimer's disease-associated E3 ubiquitin ligase HRD1"AuthorsSaito R,Kaneko M,Kitamura Y,Takata K,Kawada K,Okuma Y,Nomura Y4.Journal of proteomicsUnraveling the human dendritic cell phagosome proteome by organellar enrichment ranking."MA3-019 was used in western blot to study the protein composition of phagosomes from human dendritic cell phagosome"AuthorsBuschow SI,Lasonder E,Szklarczyk R,Oud MM,de Vries IJ,Figdor CG5.FASEB journal : official publication of the Federation of American Societies for Experimental BiologymRNA escape from stress granule sequestration is dictated by localization to the endoplasmic reticulum."MA3-019 was used in western blot to investigate the process of mRNA translation during stress response"AuthorsUnsworth H,Raguz S,Edwards HJ,Higgins CF,Yagüe E

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