PDI Monoclonal Antibody (RL90), Alexa Fluor 647
货号：MA3-019-A647
规格：50 µl
价格：4223
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：其它
荧光染料：Alexa Fluor 647
类型: | 单抗 | 同型对照： | IgG2a |
免疫原性： | Purified rat PDI protein | 用法： | 1-10 µg/mL(IF);1-10 µg/mL(ICC) |
Product Specific InformationMA3019A647 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.The MA3019A647 antigen is purified rat PDI.Target InformationThe three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| PDI Antibody (MA3-019-A647) in IFImmunofluorescent analysis of PDI (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a PDI Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-019-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
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