HSP90 beta Polyclonal Antibody

货号：PA3-012

规格：100 µg

价格：5468

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84.	用法：	1-20 µg/mL(Flow）;10-20 µg/mL(IF);10-20 µg/mL(ICC);2 µg/mL(IP);1:1000 - 1:20000(WB)

Product Specific InformationPA3-012 detects heat shock protein 90 beta (HSP90) from human, monkey, mouse, and rat tissues and cells. This antibody does not detect HSP86.PA3-012 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~84 kDa protein representing HSP84 from Hepa1 cell lysate. Immunofluorescence staining of HSP84 in Hepa1 cells with PA3-012 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP84 exists primarily as homodimers in Hepa 1 cells. Furthermore, the antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.The PA3-012 immunogen is a synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.Target InformationHeat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

HSP90 beta Antibody (PA3-012) in IFImmunofluorescent analysis of Heat Shock Protein 84 using Anti-Heat Shock Protein 84 Polyclonal Antibody (Product # PA3-012) shows staining in HepG2 Cells. Heat Shock Protein 84 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 84 (Product # PA3-012) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552, Goat Anti-Rabbit). Images were taken at 60X magnification. HSP90 beta Antibody (PA3-012) in IHCImmunohistochemistry was performed on normal biopsies of deparaffinized Human placenta tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Heat Shock Protein 84 (Product # PA3-012) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. HSP90 beta Antibody (PA3-012) in FlowFlow cytometry analysis of HSP90 was done on HeLa cells. Cells were fixed, permeabilized and stained with a HSP90 rabbit polyclonal antibody (Product # PA3-012, blue histogram) or a rabbit IgG isotype control (Product # MA5-16384, black histogram) at a dilution of 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27040) at a dilution of 1:50 for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

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参考文献：

1.International journal of cancerVacuolar H+ ATPase expression and activity is required for Rab27B-dependent invasive growth and metastasis of breast cancer."PA3-012 was used in western blot to study the mechanism by which vacuolar H(+)-ATPase regulates Rab27B-mediated invasion and metastases in estrogen receptor-positive breast cancer"AuthorsHendrix A,Sormunen R,Westbroek W,Lambein K,Denys H,Sys G,Braems G,Van den Broecke R,Cocquyt V,Gespach C,Bracke M,De Wever O2.Journal of the National Cancer InstituteEffect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis."PA3-012 was used in western blot to investigate the role of secretory small GTPase Rab27B in breast cancer proliferation and metastasis"AuthorsHendrix A,Maynard D,Pauwels P,Braems G,Denys H,Van den Broecke R,Lambert J,Van Belle S,Cocquyt V,Gespach C,Bracke M,Seabra MC,Gahl WA,De Wever O,Westbroek W3.Biochimica et biophysica actaMutations that increase both Hsp90 ATPase activity in vitro and Hsp90 drug resistance in vivo."PA3-012 was used in western blot to investigate the consequences of point mutations in heat shock protein 90 gene"AuthorsZurawska A,Urbanski J,Matuliene J,Baraniak J,Klejman MP,Filipek S,Matulis D,Bieganowski P4.Biochimica et biophysica actaProteomic identification of differentially expressed genes in mouse neural stem cells and neurons differentiated from embryonic stem cells in vitro."PA3-012 was used in western blot to identify the differentially expressed proteins in neural stem cells and embryonic stem cells"AuthorsAkama K,Tatsuno R,Otsu M,Horikoshi T,Nakayama T,Nakamura M,Toda T,Inoue N5.FASEB journal : official publication of the Federation of American Societies for Experimental BiologyChanges of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice."PA3-012 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice"AuthorsPapp E,Száraz P,Korcsmáros T,Csermely P

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