NPM1 Monoclonal Antibody (FC-61991), Alexa Fluor 555
货号：MA3-25200-A555
规格：50 µl
价格：4070
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：其它
荧光染料：Alexa Fluor 555
类型: | 单抗 | 同型对照： | IgG1 |
免疫原性： | NPM/B23 purified from rat hepatoma. | 用法： | 1-10 µg/mL(IF);1-10 µg/mL(ICC) |
Target InformationNPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| NPM1 Antibody (MA3-25200-A555) in IFImmunofluorescent analysis of Nucleophosmin (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Nucleophosmin Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-25200-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
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