HDAC2 Polyclonal Antibody, Alexa Fluor 488
货号:PA1-861-A488
规格:50 µl
价格:4070
产品类型:流式抗体
品牌:Thermo Fisher
物种:人/小鼠/大鼠
宿主:兔
抗体亚型:IgG
荧光染料:Alexa Fluor 488
类型: | 多抗 | 同型对照: | IgG |
免疫原性: | Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. | 用法: | 1-10.0 µg/mL(ICC);1-10 µg/mL(IF) |
Product Specific InformationPA1861A488 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells.The PA1861A488 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.Target InformationHistone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
HDAC2 Antibody (PA1-861-A488) in IFImmunofluorescent analysis of HDAC-2 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC-2 Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-861-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
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