Donkey anti Goat IgG (H/L):Biotin (Rat/Mouse Adsorbed)
货号:642008
规格:1 mg
价格:2652
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:IgG
物种:山羊
宿主:驴
抗体亚型:IgG
荧光染料:Biotin
产品介绍 | | |
Donkey anti Goat IgG antibodyrecognizes the heavy and light chains of goat IgG as demonstrated by ELISA. The preparation has minimal cross reaction with rat and mouse serum proteins, however, it may react with the light chains of other goat immunoglobulins. |
产品详情 | | |
Target Species | Goat |
Species Cross-Reactivity | TargetSpecies | CrossReactivity | |
| Mouse | × | |
| Rat | × | |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG conjugated to Biotin - liquid |
Antiserum Preparation | Antisera to Goat IgG were raised by repeated immunisations of donkeys with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | Goat IgG. |
Approx. Protein Concentrations | 0.5 mg/ml |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/5,000 | 1/20,000 |
Immunohistology-Frozen | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
文献 |
1.Gerry, A.B. and Leake, D.S. (2014) Effect of low extracellular pH on NF-κB activation in macrophages.Atherosclerosis. 233: 537-44.2.Armour, K.L.et al.(2014) Clearance of Human IgG1-Sensitised Red Blood Cells In Vivo in Humans Relates to the In Vitro Properties of Antibodies from Alternative Cell Lines.PLoS One. 9: e109463. |
技术参数 产品应用 C; E
产品类型 Polyclonal Antibody
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