PSMD3 Polyclonal Antibody

货号：PA1-974

规格：100 µg

价格：5468

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic Peptide: E(513) R E Q Q D L E F A K E M A E D D D D S F P(534)	用法：	1-20 µg/mL(Flow);1:50-1:500 (ICC);1:50-1:500 (IF);1 µg/mL(WB)

Product Specific InformationPA1-974 detects proteasome 19S subunit S3 from human and mouse cells.PA1-974 has been successfully used in ICC/IF, FACS, and Western blot procedures. By Western blot, this antibody detects an ~60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract.For Western blot procedures it is necessary to transfer the protein to membrane at pH 11.PA1-974 immunizing peptide corresponds to amino acid residues 513-534 from human proteasome 19S subunit S3 protein.Target InformationProteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

PSMD3 Antibody (PA1-974) in IFImmunofluorescent analysis of Proteasome 19S Subunit S3 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Proteasome 19S Subunit S3 polyclonal antibody (Product # PA1-974) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. PSMD3 Antibody (PA1-974) in FlowFlow cytometry analysis of Proteasome 19S was done on HeLa cells. Cells were fixed, permeabilized and stained with a Proteasome 19S rabbit polyclonal antibody (Product # PA1-974, pink histogram) or a rabbit IgG isotype control (Product # MA5-16384, black histogram) at a dilution of 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A27040) at a dilution of 1:50 for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

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