TER-119 Monoclonal Antibody (TER-119), FITC

货号：11-5921-81,11-5921-82,11-5921-85

规格：50μg,100μg,500μg

价格：851,1254,2623

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

荧光染料：Alexa Fluor plus 680

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
免疫原性：		用法：	0.25 μg/test(Flow)

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.Applications Reported: The TER-119 antibody has been reported for use in flow cytometric analysis.Applications Tested: The TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.

数据

TER-119 Antibody (11-5921-82) in FlowStaining of mouse bone marrow with Anti-Mouse TER-119 FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Cells in the erythrocyte population were used for analysis.

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参考文献：

1.Nature communicationsStem cell factor is selectively secreted by arterial endothelial cells in bone marrow.2.Scientific reportsType 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis."11-5921 was used in Flow cytometry/Cell sorting to study the role of type 2 innate lymphoid cells in enhancing anti-cancer immunity and controlling metastasis."AuthorsSaranchova I,Han J,Zaman R,Arora H,Huang H,Fenninger F,Choi KB,Munro L,Pfeifer CG,Welch I,Takei F,Jefferies WA3.Nature cell biologyDifferential cytokine contributions of perivascular haematopoietic stem cell niches.4.Development (Cambridge, England)Transient loss of venous integrity during developmental vascular remodeling leads to red blood cell extravasation and clearance by lymphatic vessels.5.Biochemical and biophysical research communicationsPostnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice.

技术参数

产品应用 Flow

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