CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), FITC, eBioscience™

货号：11-0439-42,11-0439-41

规格：100 tests,25 tests

价格：1855,0

产品类型：流式抗体

品牌：eBioscience

抗原：CD43

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：eBio84-3C1 (84-3C1)

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

数据

CD43 Antibody (11-0439-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD43 FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Stem cells translational medicineThe Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.2.Biomedical reportsAberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.3.Stem cells (Dayton, Ohio)Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.4.Stem cells translational medicineEnforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.5.PloS oneRapid Proliferation and Differentiation of a Subset of Circulating IgM Memory B Cells to a CpG/Cytokine Stimulus In Vitro."17-0439 was used in Flow cytometry/Cell sorting to compare the phenotype and in vitro functional response of various immune cells in response to CpG and a cytokine cocktail."AuthorsVásquez C,Franco MA,Angel J

技术参数

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