CD117 (c-Kit) Monoclonal Antibody (2B8), PE, eBioscience™
货号：12-1171-81,12-1171-82,12-1171-83
规格：50μg,100μg,200μg
价格：964,1728,2563
产品类型：流式抗体
品牌：eBioscience
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：2B8
荧光染料：PE
| 抗体类型: | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| 免疫原性： | | 用法： | 0.125 μg/test(Flow) |
Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.Applications Reported: The 2B8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.
| 数据 |
| CD117 (c-Kit) Antibody (12-1171-82) in FlowStaining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage eFluor® 450 Cockatil (Product # 88-7772-72), Anti-Mouse Ly-6A/E (Sca-1) PerCP-Cyanine5-5 (Product # 45-5981-82) and 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD117 (c-Kit) PE (right). Lineage negative/low, viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14) were used for analysis. |
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| 参考文献： |
| 1.eLifeA stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.2.Frontiers in immunologyPositive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.3.Nature communicationsmiR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.4.Nature structural and molecular biologyDefective germline reprogramming rewires the spermatogonial transcriptome.5.eLifeSingle-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. |
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