RAB11A Antibody (3H18L5), ABfinity™ Rabbit Monoclonal

货号：700184

规格：100 µg

价格：4963

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：	IgG
免疫原性：	A peptide corresponding to amino acids 179-194 of P62491.	用法：	2.0 -5 µg (ICC);2-5 µg (IF);1 -3µg/mL(WB)

Product Specific InformationThis antibody is predicted to react with bovine, chicken, equine, mouse, primate, rat, Xenopus and zebrafish based on sequence homology.ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Target InformationRab11A is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11A mRNA has been detected in a variety of tissues including brain, testis, spleen, heart, and gastrointestinal mucosa. In the gastric fundus the Rab11A protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11A protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations. Recent studies have shown Rab11A is essential for resecretion of alpha-synuclein out of neuronal cells via endosome recycling and is required for exocytosis of discoidal/fusiform vesicles of bladder umbrella cells. Rab11A is also implicated in biogenesis of Birbeck granules and endosomal activation of the PI3K/AKT signaling pathway via G beta gamma trafficking.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

RAB11A Antibody (700184) in IFImmunofluorescent analysis of Rab11 in HeLa cells using a Rab11 recombinant rabbit monoclonal antibody (Product # 700184) at a dilution of 2.5 µg/mL in the absence of peptide (top) and presence of immunogenic peptide (bottom), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 Phalloidin (Product # A12380). Hoechst only (blue, left), AF488 signal only (green, middle) and composite image with Phalloidin (right). RAB11A Antibody (700184) in WBWestern blot analysis of Rab11 in HeLa cell lysate using a Rab11 recombinant rabbit monoclonal antibody (Product # 700184) at a dilution of 6.5 µg/mL.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.eLife Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory."700184 was used in immunohistochemistry to find that CNS-specific snx6-knockout mice exhibit deficits in spatial learning and memory, accompanied with loss of spines from distal dendrites of hippocampal CA1 pyramidal cells"AuthorsNiu Y,Dai Z,Liu W,Zhang C,Yang Y,Guo Z,Li X,Xu C,Huang X,Wang Y,Shi YS,Liu JJ2.Nature communicationsCCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL."700184 was used in western blot to analyze how WASH- and CCC-mediated endosomal sorting of LDLr is needed for regular clearance of circulating LDL"AuthorsBartuzi P,Billadeau DD,Favier R,Rong S,Dekker D,Fedoseienko A,Fieten H,Wijers M,Levels JH,Huijkman N,Kloosterhuis N,van der Molen H,Brufau G,Groen AK,Elliott AM,Kuivenhoven JA,Plecko B,Grangl G,McGaughran J,Horton JD,Burstein E,Hofker MH,van de Sluis B3.The Journal of neuroscience : the official journal of the Society for NeuroscienceExogenous α-synuclein decreases raft partitioning of Cav2.2 channels inducing dopamine release."700184 was used in western blot to test if alpha-synuclein modulates neurotransmitter release"AuthorsRonzitti G,Bucci G,Emanuele M,Leo D,Sotnikova TD,Mus LV,Soubrane CH,Dallas ML,Thalhammer A,Cingolani LA,Mochida S,Gainetdinov RR,Stephens GJ,Chieregatti E

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17783.htm