CD150 Monoclonal Antibody (mShad150), PE-Cyanine7

货号：25-1502-82

规格：100μg

价格：3603

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：mShad150

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

产品详细信息Description: The mShad150 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells, in particular Th1, and B cells; this expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. The mShad150 antibody has been reported to also stain the CD150+CD244-CD48-CD41- population of pluripotent hematopoietic stem cells.Applications Reported: This mShad150 antibody has been reported for use in flow cytometric analysis.Applications Tested: This mShad150 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells.Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).

数据

CD150 Antibody (25-1502-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (left) or 0.25 µg of Anti-Mouse CD150 PE-Cyanine7 (right). Total viable cells were used for analysis.Rat IgG2b kappa Isotype Control (eB149/10

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参考文献：

1.OncotargetRecipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.2.PloS oneSmall molecule p300/catenin antagonist enhances hematopoietic recovery after radiation.3.Nature communicationsSleep disruption impairs haematopoietic stem cell transplantation in mice."25-1502 was used in Flow cytometry/Cell sorting to provide insights into cellular and molecular mechanisms underlying the effects of sleep deprivation on HSCs."AuthorsRolls A,Pang WW,Ibarra I,Colas D,Bonnavion P,Korin B,Heller HC,Weissman IL,de Lecea L4.BloodGlypican-3-mediated inhibition of CD26 by TFPI: a novel mechanism in hematopoietic stem cell homing and maintenance."25-1502 was used in Flow cytometry/Cell sorting to present a novel role for TFPI and GPC3 in regulating haematopoietic stem cell migration."AuthorsKhurana S,Margamuljana L,Joseph C,Schouteden S,Buckley SM,Verfaillie CM

技术参数

产品应用 Flow

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