Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 488

货号：53-4321-80

规格：25μg

价格：987

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBR2a

荧光染料：Alexa Fluor 488

抗体类型:		同型对照：	Ig2a, kappa
免疫原性：		用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)；Assay-Dependent (IF);Assay-Dependent(ICC)

产品详细信息Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: The Alexa Fluor® 488 Rat IgG2a Isotype Control has been reported useful as an isotype control for immunofluorescent staining, flow cytometric analysis, immunocytochemistry, and immunohistochemistry.Applications Tested: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.Frontiers in systems neuroscienceTraumatic Brain Injury Upregulates Phosphodiesterase Expression in the Hippocampus."53-4321 was used in Flow cytometry/Cell sorting to identify several possible PDI targets for reducing hippocampal synaptic plasticity deficits and improve cognitive function acutely after Traumatic brain injury (TBI)."AuthorsWilson NM,Titus DJ,Oliva AA,Furones C,Atkins CM2.Nature immunologyThe adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation."53-4321 was used in Flow cytometry/Cell sorting to study the behaviour of ASC specks and their contribution to IL-1β - induced inflammation."AuthorsFranklin BS,Bossaller L,De Nardo D,Ratter JM,Stutz A,Engels G,Brenker C,Nordhoff M,Mirandola SR,Al-Amoudi A,Mangan MS,Zimmer S,Monks BG,Fricke M,Schmidt RE,Espevik T,Jones B,Jarnicki AG,Hansbro PM,Busto P,Marshak-Rothstein A,Hornemann S,Aguzzi A,Kastenmüller W,Latz E3.Cancer researchThe CD4(+) T-cell response of melanoma patients to a MAGE-A3 peptide vaccine involves potential regulatory T cells."53-4321 was used in Immunocytochemistry to investigate the CD4+ T-cell response of melanoma patients to a MAGE-A3 peptide vaccine."AuthorsFrançois V,Ottaviani S,Renkvist N,Stockis J,Schuler G,Thielemans K,Colau D,Marchand M,Boon T,Lucas S,van der Bruggen P4.Cancer researchThe CD4(+) T-cell response of melanoma patients to a MAGE-A3 peptide vaccine involves potential regulatory T cells."53-4321 was used in Immunocytochemistry to investigate the CD4+ T-cell response of melanoma patients to a MAGE-A3 peptide vaccine."AuthorsFrançois V,Ottaviani S,Renkvist N,Stockis J,Schuler G,Thielemans K,Colau D,Marchand M,Boon T,Lucas S,van der Bruggen P

技术参数

产品应用 Flow;ICC

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