OCT4 Monoclonal Antibody (9B7), HRP

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OCT4 Monoclonal Antibody (9B7), HRP

货号: MA1-104-HRP

规格:100 µL

价格:3971

产品类型:免疫组化一抗

品牌:Thermo Fisher

抗原:OCT4

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:9B7

荧光染料:HRP

类型:

单抗

同型对照:

IgG

免疫原性:

Full length human recombinant protein of human POU5F1 produced in E.coli.

用法:

1:250-1:1000(WB)

Product Specific InformationMA1-104-HRP detects Oct4/Pou5F1 in human embryonic carcinoma cells.By Western blot, this antibody detects a band at 40 kDa in human embryonal carcinoma cells, but not in negative control cells.Target InformationOct4 is a transcription factor which plays a key role in embryonic development, especially during early embryogenesis. Oct4 is one of the four Yamaka factors, necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

OCT4 Antibody (MA1-104-HRP) in WB

Western blot analysis of Oct4/Pou5F1 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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