TRA-1-60 Monoclonal Antibody (TRA-1-60), PE

货号： MA1-023-PE

规格：100 µL

价格：4006

产品类型：流式抗体

品牌：Thermo Fisher

抗原：TRA-1-60

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-60

荧光染料：PE

类型:	单抗	同型对照：	IgM
免疫原性：	Human embryonal carcinoma cell line 2102Ep.	用法：	1:50-1:500(Flow)

Product Specific InformationMA1-023-PE has been successfully used in ICC/IF and flow cytometry applications on human samples.Target InformationTRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-60 Antibody (MA1-023-PE) in FlowFlow cytometry analysis of TRA-1-60 in NCCIT (red filled histogram) and HeLa cells (red unfilled histogram) compared to unstained cells (blue histogram). Positive staining is observed on NCCIT cells when compared to no antibody control (middle panel) and to TRA-1-60-negative HeLa cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature, and incubated with a PE-conjugated TRA-1-60 monoclonal antibody (Product # MA1-023-PE) in 2% BSA-PBS at a dilution of 1:100 for 60 minutes at room temperature. Cells were washed and re-suspended in PBS for FACS analysis.

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参考文献：

1.Stem cells translational medicine Quantification of Etoposide Hypersensitivity: A Sensitive, Functional Method for Assessing Pluripotent Stem Cell Quality.

技术参数

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