TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience™

货号：14-8883-82, 14-8883-80

规格：100ug,25ug

价格：2902,0

产品类型：流式抗体

品牌：eBioscience

抗原：TRA-1-81

物种：人/小鼠

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-81

荧光染料：其它

类型:	单抗	同型对照：	IgM
免疫原性：		用法：	0.5 µg/test(Flow);5 µg/mL(WB)

Product Specific InformationDescription: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.Applications Tested: This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonic carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). This antibody has been tested by immunofluorescent staining of formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-81 (Podocalyxin) Antibody (14-8883-82) in ICCImmunocytochemistry of human embryonic stem cells using 5 µg/mL Anti-Human TRA-1-81 Purified followed by Anti-Mouse TRITC (Red).Nuclei are stained with 10 µg/mL Anti-OCT3/4 Alexa Fluor® 488 (Product # 53-5841-82) Data courtesy of Zeng lab at the Buck Institute. TRA-1-81 (Podocalyxin) Antibody (14-8883-82) in FlowStaining of 2102Ep human embryonic carcinoma cell line with 0.25 µg of Mouse IgM Isotype Control Purified (Product # 14-4752-82) (open histogram) or 0.25 µg of Anti-Human TRA-1-81 Purified (filled histogram) followed by Anti-Mouse IgM PE (Product # 12-5890-82).Total viable cells were used for analysis.

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参考文献：

1.PloS one Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach. "14-8883 was used in Immunofluorescence to establish efficient nonviral reprogramming protocols for the derivation of stable marmoset monkey iPS cells with a six-factor-in-one-construct approach." AuthorsDebowski K,Warthemann R,Lentes J,Salinas-Riester G,Dressel R,Langenstroth D,Gromoll J,Sasaki E,Behr R 2.Stem cell reviews PRMT5 is required for human embryonic stem cell proliferation but not pluripotency. "14-8883 was used in Flow cytometry/Cell sorting to evaluate the role of protein arginine methyltransferase 5 in human embryonic stem cell self-renewal and pluripotency." AuthorsGkountela S,Li Z,Chin CJ,Lee SA,Clark AT 3.Orphanet journal of rare diseases Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation. "14-8883 was used in Immunocytochemistry to investigate the cellular mechanisms behind fibrodysplasia ossificans progressiva using a novel human induced pluripotent stem cell disease model." AuthorsMatsumoto Y,Hayashi Y,Schlieve CR,Ikeya M,Kim H,Nguyen TD,Sami S,Baba S,Barruet E,Nasu A,Asaka I,Otsuka T,Yamanaka S,Conklin BR,Toguchida J,Hsiao EC

技术参数

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