TRA-1-81 Monoclonal Antibody (TRA-1-81)

货号：MA1-024,MA1-024X

规格：100 ug,20 uL

价格：4070,2148

产品类型：流式抗体

品牌：Thermo Fisher

抗原：TRA-1-81

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-81

荧光染料：其它

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Beta-tubulin from sea urchin (S. purpuratus) sperm.	用法：	2.0 µg/mL(ICC);10 µg/mL(IF);1 µg/mL(WB)

Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-81 Antibody (MA1-024) in IFImmunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing TRA-1-81 (Product # MA1-024), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with goat-anti-mouse IgM secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. TRA-1-81 Antibody (MA1-024) in FlowFlow cytometry analysis of TRA-1-81 was performed on NCCIT cells. NCCIT cells were harvested, fixed and washed with PBS. Cells were incubated with 0.4 µg of TRA-1-60 monoclonal antibody (Product # MA1-024), followed by incubation with FITC-conjugated goat-anti-mouse IgM secondary antibody (Product # 31992), indicated by the shifted peak (blue line). To control for non-specific binding, cells were also incubated with secondary antibody alone (black line). Incubations were performed for 30 mins in the dark and 10,000 cells were analyzed for each sample. TRA-1-81 Antibody (MA1-024) in IFImmunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product # MA1-024) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:20 overnight at 4 °C, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification. TRA-1-81 Antibody (MA1-024) in WBWestern blot analysis of TRA-1-81 was performed by loading 100 µg of NTERA-2 whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgM HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product # 34075).

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