TRA-1-81 Monoclonal Antibody (TRA-1-81), DyLight 550
货号：MA1-024-D550
规格：100 µL
价格：4070
产品类型：免疫组化一抗
品牌：Thermo Fisher
抗原：TRA-1-81
物种：人
宿主：小鼠
抗体亚型：IgM
克隆号：TRA-1-81
荧光染料：DyLight 550
类型: | 单抗 | 同型对照： | IgM |
免疫原性： | human embryonal carcinoma cell line 2102Ep | 用法： | 1:10-1:100(ICC);1:10-1:100(IF) |
Product Specific InformationMA1-024-D550 has been successfully used in ICC/IF applications on human samples.Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| TRA-1-81 Antibody (MA1-024-D550) in IFImmunofluorescent analysis of TRA-1-81 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-D550) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification. |
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参考文献： |
| 1.Journal of cellular physiology PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons. |
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