Nanog Monoclonal Antibody (23D2-3C6)

货号：MA1-017

规格：100 µg

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

抗原：Nanog

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：23D2-3C6

荧光染料：其它

类型:	单抗	同型对照：	IgG1
免疫原性：	Full-length human recombinant protein expressed in bacteria	用法：	1:50 -1:100(ICC);1:50 -1:100(IF);1:500 - 1:2000(WB)

Product Specific InformationWestern blot analysis of MA1-017 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. MA1-017 shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (MA1-017) in IFImmunofluorescent analysis of Nanog (green) in NTERA-2 and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody (Product # MA1-017) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502). F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. Nanog Antibody (MA1-017) in FlowFlow cytometric analysis of Nanog (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37°C. Cells were incubated with a Nanog monoclonal antibody (Product # MA1-017) or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500 µL of FACS buffer containing 10 µL of 4% paraformaldehyde, and analyzed on a flow cytometer. Nanog Antibody (MA1-017) in IFImmunofluorescent analysis of Nanog (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody (Product # MA1-017) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.

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参考文献：

1.Stem cell researchGeneration of an iPSC line from a patient with GTP cyclohydrolase 1 (GCH1) deficiency: HDMC0061i-GCH1."MA1017 was used in immunocytochemistry to report the first model system to investigate the pathomechanism underlying GTP cyclohydrolase 1 mutations"AuthorsJung-Klawitter S,Ebersold J,Göhring G,Blau N,Opladen T2.Stem cell researchGeneration of an iPSC line from a patient with tyrosine hydroxylase (TH) deficiency: TH-1 iPSC."MA1017 was used in immunocytochemistry to generate and characterize tyrosine hydroxylase-expressing iPSCs"AuthorsJung-Klawitter S,Blau N,Sebe A,Ebersold J,Göhring G,Opladen T3.Stem cell reportsConditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation."MA1-017 was used in immunocytochemistry to demonstrate that dCas9 activator controls human pluripotent stem cell differentiation into endodermal lineages"AuthorsBalboa D,Weltner J,Eurola S,Trokovic R,Wartiovaara K,Otonkoski T4.Nucleic acids researchConservative site-specific and single-copy transgenesis in human LINE-1 elements."MA1-017 was used in western blot to discuss Phage lambda integrase as a transgenesis tool"AuthorsVijaya Chandra SH,Makhija H,Peter S,Myint Wai CM,Li J,Zhu J,Ren Z,D'Alcontres MS,Siau JW,Chee S,Ghadessy FJ,Dröge P5.PLoS geneticsAssociation of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes."MA1-017 was used in western blot to test if lncRNA SRA interacts with TrxG or PRC2"AuthorsWongtrakoongate P,Riddick G,Fucharoen S,Felsenfeld G

技术参数

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